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Date: 20 August 2004
| Rank | Name | Nation | Time |
| 1 | Anna Meares | AUS | 33.952 |
| 2 | Jiang Yonghua | CHN | 34.112 |
| 3 | Natallia Tsylinskaya | BLR | 34.167 |
| 4 | Simona Krupeckaite | LTU | 34.317 |
| 5 | Yvonne Hijgenaar | NED | 34.532 |
| 6 | Victoria Pendleton | GBR | 34.626 |
| 7 | Lori-Ann Muenzer | CAN | 34.628 |
| 8 | Nancy Contreras | MEX | 34.783 |
| 9 | Svetlana Grankovskaya | RUS | 34.797 |
| 10 | Sayuri Osuga | JPN | 35.045 |
| 11 | Katrin Meinke | GER | 35.088 |
| 12 | Tamilla Abassova | RUS | 35.147 |